Fecha/hora: 01/06/2024 10:24:36

Biblioteca(s): 	INIA La Estanzuela.	
Fecha corriente: 	13/09/2017	
Fecha de la última actualización: 	06/12/2018	
Autoria: 	BERTON, MP.; DE OLIVEIRA SILVA, RM.; PERIPOLLI, E.; STAFUZZA ,NB.; MARTIN, JF.; ÁLVAREZ, MS.; GAVINÃ, BV.; TORO, MA.; BANCHERO, G.; OLIVEIRA ,PS.; ELER, JP.; BALDI, F.; FERRAZ, JB.	
Afiliación: 	Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal,  Brazil.; Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, Brazil.; Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, Brazil.; Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, Brazil.; Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA, Madrid, España.; Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA, Madrid, España.; Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA, Madrid, España.; Departamento de Producción Agraria, School of Agricultural, Food and Byosystems Engineering, Universisdad Politécnica de Madrid, España.; GEORGGET ELIZABETH BANCHERO HUNZIKER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Faculdade de Zootecnia e Engenharia de Alimentos, Nucleo de Apoio à Pesquisa em Melhoramento Animal, Biotecnologia e Transgenia, Universidade de São Paulo, Brasil.; Faculdade de Zootecnia e Engenharia de Alimentos, Nucleo de Apoio à Pesquisa em Melhoramento Animal, Biotecnologia e Transgenia, Universidade de São Paulo, Brasil.; FERNANDO BALDI, Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Brazil.; Faculdade de Zootecnia e Engenharia de Alimentos, Nucleo de Apoio à Pesquisa em Melhoramento Animal, Biotecnologia e Transgenia, Universidade de São Paulo,Brasil.	
Título: 	Genomic regions and pathways associated with gastrointestinal parasites resistance in Santa Inês breed adapted to tropical climate.	
Año de publicación: 	2017	
Fuente/Imprenta: 	Journal of Animal Science and Biotechnology , v.8.p. 73.OPEN ACCESS.	
Descripción Física: 	Received: 7 December 2016 Accepted: 12 June 2017 Published: 4 September 2017.	
DOI: 	10.1186/s40104-017-0190-4	
Idioma: 	Inglés	
Notas: 	Article history: Received: 7 December 2016 //Accepted: 12 June 2017 //Published: 4 September 2017.   This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.	
Contenido: 	Abstract
Background: The aim of this study was to estimate variance components and to identify genomic regions and
pathways associated with resistance to gastrointestinal parasites, particularly Haemonchus contortus, in a breed of
sheep adapted to tropical climate. Phenotypes evaluations were performed to verify resistance to gastrointestinal
parasites, and were divided into two categories: i) farm phenotypes, assessing body condition score (BCS), degree
of anemia assessed by the famacha chart (FAM), fur score (FS) and feces consistency (FC); and ii) lab phenotypes,
comprising blood analyses for hematocrit (HCT), white blood cell count (WBC), red blood cell count (RBC),
hemoglobin (HGB), platelets (PLT) and transformed (log10) egg per gram of feces (EPGlog). A total of 576 animals
were genotyped with the Ovine SNP12k BeadChip (Illumina, Inc.), that contains 12,785 bialleleic SNP markers. The
variance components were estimated using a single trait model by single step genomic BLUP procedure.
Results: The overall linkage disequilibrium (LD) mean between pairs of markers measured by r
2 was 0.23. The overall
LD mean between markers considering windows up to 10 Mb was 0.07. The mean LD between adjacent SNPs across
autosomes ranged from 0.02 to 0.10. Heritability estimates were low for EPGlog (0.11), moderate for RBC (0.18), PLT (0.
17) HCT (0.20), HGB (0.16) and WBC (0.22), and high for FAM (0.35). A total of 22, 21, 23, 20, 26, 25 and 23 windows for
EPGlog for FAM, WBC, RBC, PLT, HCT and HGB traits were identified, respectively. Among the associated windows, 10
were shown to be common to HCT and HGB traits on OAR1, OAR2, OAR3, OAR5, OAR8 and OAR15.
Conclusion: The traits indicating gastrointestinal parasites resistance presented an adequate genetic variability to
respond to selection in Santa Inês breed, and it is expected a higher genetic gain for FAM trait when compared to the
others. The level of LD estimated for markers separated by less than 1 Mb indicated that the Ovine SNP12k BeadChip
might be a suitable tool for identifying genomic regions associated with traits related to gastrointestinal parasite
resistance. Several candidate genes related to immune system development and activation, inflammatory response,
regulation of lymphocytes and leukocytes proliferation were found. These genes may help in the selection of animals
with higher resistance to parasites.	
Palabras-Clave: 	GWAS; LINKAGE DISEQUILIBRIUM; PARASITES RESISTANCE; RESISTENCIA A PARÁSITOS GASTROINTESTINALES; SANTA INES BREED.	
Thesagro: 	ENFERMEDADES DE LOS ANIMALES.	
Categoría del tema: 	L73 Enfermedades de los animales	
URL: 	http://www.ainfo.inia.uy/digital/bitstream/item/12142/1/Berton-MP.-2017.-Jr.-Animal-Sc.-Biotech..pdf	
Registro original: 	INIA La Estanzuela (LE)	
Ejemplares: 	LE - (102192-1) - PXI - AP - DD -  -  -  - DIS